アプリケーショントピックス 37種類

FRET解析(CFP-YFP)例

・ CFP-YFP transformed E.Coli
- The CFP-YFP FRET pair is linked with an 8 aa peptide.
-
FRET of E.Coli transformed with the CFP-YFP construct can be detected with a Tecan Microplate Reader.
- Controls: negative (empty vector), CFP and YFP alone
・ CFP
- Excitation (434nm)
・ Arc Lamp: 425/40Filter
- Emission (477nm)
・ 480/40 emission filter (FL1)
・ YFP
- Excitation (513nm)
・ 488nm laser
・ FRET from CFP (488 nm laser off)
- Emission (527 nm)
・ 535/30 emission filter (FL2)

FRET解析 Cell Lab Quanta SC を使用して、435nm励起光を照射し、2カラー測定


FL1 (CFP) FL2 (YFP)


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