Thomas RA, Krishan A, Robinson DM, Sams C and Costa F: NASA/American Cancer Society high-resolution flow cytometry project-I. Cytometry 2001, 43:2-11.

Note: One of the original publications describing basic principle of the original Quanta Flow Cytometer.

Krishan A, Wen J, Thomas RA, Sridhar KS, Smith Jr. WI: NASA/American Cancer Society high-resolution flow cytometry project-III. Multiparametric analysis of
DNA content and electronic nuclear volume in human solid tumors. Cytometry 2001, 43:16-22

Note: One of the original publications describing basic principle of the original Quanta Flow Cytomete.

Yoshida N and Saeki Y: Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA. Appl Microbiol Biotechnol. 2004, 65: 566-575

Application: Identification of chrysotile aggregates by accurately measuring particle size (electronic volume) and 4’,6-diamidino-2-phenylindole (DAPI) signal.

Krishan A and Cabana R: Flow Cytometric analysis of electronic nuclear volume and DNA content in normal mouse tissues. Cell Cycle 2004, 3: 380-383

Application: Cell cycle analysis with nuclear isolation medium (NIM)-DAPI and accurate nuclear volume determination by Electronic Volume (EV) measurement.

Kadota Y, Watanabe T, Fujii S, Maeda Y, Ohno R, Higashi K, Sano T, Muto S, Hasezawa S and Kuchitsu K: Cell cycle dependence of elicitor-induced signal transduction in tobacco BY-2 Cells. Plant Cell Physiol. 2005, 46: 156-165.

Application: Cell cycle analysis with NIM.

Krishan A and Dandekar, RD: DAPI Fluorescence in nuclei isolated from tumors. J Histochem Cytochem. 2005, 53, 1033-1036

Application: Cell cycle analysis with NIM-DAPI.

Weinberger LS, Burnett JC, Toettcher JE,2 Arkin AP and Schaffer DV: Stochastic gene expression in a lentiviral positive-feedback loop: HIV-1 Tat fluctuations drive phenotypic diversity. Cell 2005, 122: 169-182 (data in Supplemental Data section).
Applications: Detection of GFP expression. Cell size measurement by EV. Cell cycle analysis with NIM-DAPI. Cell cycle analysis and nuclei size measurement on GFP expressed cells.

Note: The paper described a very interesting phenomenon. After NIM (a solution contained NP-40) treatment, 2% formaldehyde fixed GFP expressed Jurkat cells display GFP fluorescence equivalent to unfixed, non-NIM-treated cells. However, the fixed and NIM-treated cells have the same volume measurement as bare nuclei (~5 μm diameter) because formaldehyde fixation plus NIM-lysing still permits ions to infuse the fixed cytoplasm but not the nucleus. Therefore, fixed GFP expressed cell with NIM treatment maintains a halo of GFP around the major volume determinant, i.e. nucleus. This observation allowed the authors to design the experiment to determine correlation of aneuploidy with GFP expression.

Lefurgey A, Gannon, Blum J and Ingram P: Leishmania donovani amastigotes mobilize organic and inorganic osmolytes during regulatory volume decrease. J Eukaryot Microbiol. 2005, 52: 277-289

Application: Accurate cell size measurement by Electronic Volume (EV).

Furie B, Zwicker J, Larocca T, Kos C, Bauer B and Furie BC: Tissue factor-bearing microparticles and cancer-associated thrombosis. Haematologica reports 2005,1(9):5-8

Application: Determine of size, size distribution, and concentration of tissue factor-bearing microparticles.

Horton JK, Donna F. Stefanick DF, Naron JM, Kedar PS, and Wilson SH: Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure. J Biol Chem. 2005, 280: 15773-15785,

Application: Cell cycle analysis with NIM-DAPI.

Krishan A, Dandekar P, Nathan N, Hamelik R, Miller C and Shaw J: DNA index, genome size, and electronic nuclear volume of vertebrates from the Miami Metro Zoo. Cytometry Part A, 2005, 65A:26-34

Application: Determination of DNA content and genome size with NIM-DAPI. Accurate nuclear volume measurement by Electronic Volume (EV).

Aparicio-Fernandez X, Garcia-Gasca T, Yousef GG, Lila MA, Gonzalez de Mejia E, and Loarca-pina G: Chemopreventive activity of polyphenolics from black Jamapa Bean ( Phaseolus vulgaris L.) on HeLa and HaCaT Cells. J Agric Food Chem. 2006, 54, 2116--2122

Application: HeLa cell cycle analysis with NIM-DAPI.

Krishan A, Ganjei-Azar P, Jorda M, Hamelik RM, Reis IM and Nadji M: Detection of tumor cells in body cavity fluids by flow cytometric and immunocytochemical analysis. Diag Cytopathol. 2006, 34: 528-541

Application: High resolution DNA and nuclear volume measurement.

Bauer S, Yu LK, Demetri GD, and Fletcher JA: Heat shock protein 90 inhibition in imatinib-resistant gastrointestinal stromal tumor. Cancer Res. 2006, 66: 9153-9161

Application: Cell cycle analysis with NIM-DAPI.

Hess-Wilson JK, Daly HK, Zagorski WA, Montville CP, Knudsen KE: Mitogenic action of the androgen receptor sensitizes prostate cancer cells to Taxane-based cytotoxic insult. Cancer Res. 2006, 66: 11998-12008

Application: Cell cycle analysis with propidium iodide (PI) staining.

Kaddour-Djebbar I, Lakshmikanthan V, Shirley RB,1 Ma Y, Ronald W. Lewis RW, and Kumar MV: Therapeutic advantage of combining calcium channel blockers and TRAIL in prostate cancer. Mol Cancer Ther. 2006, 5, 1958-1966

Application: Apoptosis analysis with Annexin V-FITC and 7-amino-actinomycin D (7-AAD) staining.

McGuire MJ, Samli KN, Chang YC, and Brown KC: Novel ligands for cancer diagnosis: Selection of peptide ligands for identification and isolation of B-cell lymphomas. Exp Hematol. 2006, 34: 443-452

Application: Detection of biotinylated peptide binding to cell surface with streptavidin- Phycoerythrin (PE).

Cabana R, Frolova EG, Kapoor V, Thomas RA, Krishan A, Telford WG: The minimal instrumentation requirements for Hoechst side population analysis: Stem cell analysis on low-cost flow cytometry platforms. Stem Cells 2006, 24: 2573-2581

Application: Stem cell side population analysis.

Miyazaki H, Shiozaki A, Niisato N and Marunaka Y: Physiological significance of hypotonicity-induced regulatory volume decrease: reduction in intracellular Cl- concentration acting as an intracellular signaling. Am J Physiol Renal Physiol.2007; 292: F1411-F1417
Applications: Accurate cell size measurement by Electronic Volume (EV). Intracellular Cl- concentration ([Cl-]i) measurement by a halide-specific fluorescent dye, N-(6-methoxyquinolyl) acetoethyl ester (MQAE).

Note: It is the first publication to demonstrate significance of normalizing fluorescence with cell volume (EV), i.e. fluorescence concentration (FC), in measuring [Cl-]i.

Xie L, Li Q, Johnson J, Zhang J, Milhous W and Kyle D: Development and validation of flow cytometric measurement for parasitaemia using autofluorescence and YOYO-1 in rodent malaria. Parasitol. 2007, Apr 20: 1-12.

Application: Fast, highly sensitive, reproducible and accurate measurement for parasitaemia.

Zagorski WA, Knudsen ES, Reed MF: Retinoblastoma deficiency increases chemosensitivity in lung cancer. Cancer Res. 2007;67: 8264-73

Applications: Cell cycle analysis with propidium iodide (PI) staining;Bromodeoxyuridine (BrdUrd) incorporation

Sandhu KS, Naciri M, Al-Rubeai M: Prediction of recombinant protein production in an insect cell baculovirus system using a flow cytometric technique. Journal of Immunological Methods 2007; 325, 104-113.

Applications: Cell size and granularity measurement; AmCyan fluorescent protein detection.

Maki M, Miyazaki H, Nakajima K, Yamane J, Niisato N, Morihara T, Kubo T, Marunaka Y: Chloride-dependent acceleration of cell cycle via modulation of Rb and cdc2 in osteoblastic cells. Biochem and Biophys Res Comm 2007; 361: 1038-1043

Applications: Cell cycle analysis with NIM-DAPI