FRET解析（CFP-YFP）例

• ･ CFP-YFP transformed E.Coli
• - The CFP-YFP FRET pair is linked with an 8 aa peptide.
• - FRET of E.Coli transformed with the CFP-YFP construct can be detected with a Tecan Microplate Reader.
• - Controls: negative (empty vector), CFP and YFP alone

• ･ CFP
• - Excitation (434nm)
• ･ Arc Lamp: 425/40Filter
• - Emission (477nm)
• ･ 480/40 emission filter (FL1)

• ･ YFP
• - Excitation (513nm)
• ･ 488nm laser
• ･ FRET from CFP (488 nm laser off)
• - Emission (527 nm)
• ･ 535/30 emission filter (FL2)